#Text on image for mac update#

In this case, use the original file’s spatial calibration (located under Image › Properties) to update the Image › Properties values of the final, processed image. Sometimes the spatial calibration may be lost after applying an image processing function. The “ Microscope Scale” plugin is another alternative and can be customized using a drop-down box of objectives to apply spatial calibrations. Spatial calibration can also be found and set in the Image › Properties dialog. Then the calibration can be applied using Analyze › Set Scale ( see below). These settings include camera binning, frame size, objective, confocal zoom, etc. In the case that the files have no calibration, you can apply the calibration using images of a stage micrometer with the same settings as your experiment. Zeiss zvi’s exported as TIFFs) will lose their calibration information completely.

Perkin Elmer) are not calibrated while some “exported” image files (e.g. However, it is important to keep in mind that some file formats (e.g. Some microscope models (e.g., Biorad PIC, and Zeiss LSM confocal image files) will use the zoom and objective magnification settings for internal calibration. Note that the units “um” will automatically change to “µm”. Spatial calibrationĪn image’s spatial calibration can be edited using Image › Properties. A standard size should be used for the scale bars on all images if possible to help avoid confusion. All images for publications should include a scale bar.

Text on image for mac